{"id":1223,"date":"2017-04-05T13:54:10","date_gmt":"2017-04-05T10:54:10","guid":{"rendered":"http:\/\/polymerjournal.kiev.ua\/en\/?page_id=1223"},"modified":"2017-10-23T21:31:31","modified_gmt":"2017-10-23T18:31:31","slug":"sergeyeva_satyr","status":"publish","type":"page","link":"http:\/\/polymerjournal.kiev.ua\/en\/sergeyeva_satyr\/","title":{"rendered":"2016 (3) 10"},"content":{"rendered":"<p><a href=\"https:\/\/doi.org\/10.15407\/polymerj.38.03.261\">https:\/\/doi.org\/10.15407\/polymerj.38.03.261<\/a><\/p>\n<p><strong>Biosensor system for detection of bisphenol\u00a0A in aqueous solutions<\/strong><\/p>\n<p><em>\u00a0<\/em><\/p>\n<p><strong><em>T.A. Sergeyeva<\/em><\/strong><strong><em>, A.V. Satyr<\/em><\/strong><strong><em>, O.V. Piletska<\/em><\/strong><strong><em>, L.A. Gorbach<\/em><\/strong><strong><em>, <\/em><\/strong><strong><em>\u041e<\/em><\/strong><strong><em>.<\/em><\/strong><strong><em>\u041e<\/em><\/strong><strong><em>. Brovko<\/em><\/strong><strong><em>, A.V. El\u2019skaya<\/em><\/strong><\/p>\n<p>&nbsp;<\/p>\n<p>Institute of Molecular Biology and Genetics NAS of Ukraine<\/p>\n<p>150 Zabolotnogo str., Kyiv, 03680, Ukraine<\/p>\n<p>University of Leicester<\/p>\n<p>Leicester\u00a0LE1\u00a07RH, United Kingdom<\/p>\n<p>Institute of Macromolecular Chemistry NAS of Ukraine<\/p>\n<p>48, Kharkivske shose, Kyiv, 02160, Ukraine<\/p>\n<p>&nbsp;<\/p>\n<p>Polym. J., 2016, <strong>38<\/strong>, no. 3: 261-266.<\/p>\n<p>&nbsp;<\/p>\n<p>Section: Synthesis polymers.<\/p>\n<p>&nbsp;<\/p>\n<p>Language: Ukrainian.<\/p>\n<p>&nbsp;<\/p>\n<p>Abstract:<\/p>\n<p style=\"padding-left: 90px;\"><em>Molecularly imprinted polymer membranes based on acrylate-polyurethane semi-IPNs capable of highly-selective recognition of bisphenol A were synthesized. Composition of the MIP membranes was optimized using the method of computational modelling (molecular dynamics). The synthesized membranes were used as a basis of simple and inexpensive colorimetric sensor system for detection of bisphenol A in aqueous solutions. Selective recognition of bisphenol A by the MIP membranes is provided due to formation of selective receptor sites in their structure, which are complementary to the analyte molecules. Molecules of bisphenol A, which were adsorbed by the surface of the MIP membranes, were revealed using colored reaction with 4-aminoantipyrine. Intensity of the membranes\u2019 staining was proportional by bisphenol A concentration in the analyzed sample. Detection limit for bisphenol A detection with the developed colorimetric sensor system comprised 50\u00a0\u00b5M, while the detection range was 50\u00a0\u00b5M \u2013 1\u00a0mM. The developed sensor systems were tested for environmental monitoring (analysis of natural waters).<\/em><\/p>\n<p><strong>Key words: <\/strong>molecularly imprinted polymers, membranes, sensors, biosensors, sensor systems, bisphenol\u00a0A.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>References<\/strong><\/p>\n<ol>\n<li><em>1. Geens T., Goeyens L., Covaci A.<\/em>, Are potential sources for human exposure to bisphenol-A overlooked? , Inter. J. of Hygiene and Environmental Health, 2011, <strong>214<\/strong>: 339-347.<\/li>\n<li><em>2. Michalovicz J.<\/em> Bisphenol A \u2013 Sources, toxicity and biotransformation. Environmetal toxicology and pharmacology, 2014, Vol. 37: 738\u2013758.<\/li>\n<li><em>3. Rubin B.S.<\/em> Bisphenol A: an endocrine disruptor with widespread exposure and multiple effects. J. Steroid. Biochem. Mol. Biol, 2011, <strong>127<\/strong>, no. 1-2:\u00a027-34.<\/li>\n<li><em>4. Ballesteros-Gomez A., Rubio S., Perez-Bendito D.<\/em> Analytical methods for the determination of bisphenol A in food. J. Chromatogr, 2009, <strong>1216<\/strong>, no. 3: 449-469.<\/li>\n<li><em>5. Ju C., Xiong Y., Gao A., Yang <\/em><em>\u0422<\/em><em>., Wang L.<\/em> Development of a direct competitive enzyme-linked immunosorbent assay using a sensitive monoclonal antibody for Bisphenol A. Hybridoma, 2011, <strong>30<\/strong>, no. 1: 95-100,.<\/li>\n<li><em>6. Kaddar N., Bendridi N., Harthe C., de Revel M. R., Bienvenu A.-L., Cbilleron C.-Y., Mappus E., Pugeat M., Dechaud H.<\/em> Development of a radioimmunoassay for the measurement of Bisphenol A in biological samples. Anal. Chim. Acta, 2009,<strong> 645<\/strong>, no. 1-2: 1-4.<\/li>\n<li><em>7. Ullah R., Li M., Zhu Y.-M.<\/em>, Terahertz and FTIR spectroscopy of \u201cBisphenol A\u201d. J. Mol. Struct, 2014, <strong>1059<\/strong>: 255-259.<\/li>\n<li><em>8. Dekant W., Volkel W.<\/em> Human exposure to bisphenol A by biomonitoring: Methods, results and assessment of environmental exposures. Toxicol. Appl. Pharmacol, 2008, <strong>228<\/strong>, no. 1: 114-134.<\/li>\n<li><em>9. Ragavan K., Pastori N. K., Thakur M. S.<\/em> Sensors and biosensors for analysis of bisphenol-A. Trends Anal. Chem, 2013, Vol. 52: 248-260.<\/li>\n<li><em>10. Sergeyeva T.A.,Slinchenko O.A., Gorbach L.A.,<\/em><em>Matyushov V.F.,\u00a0Brovko O.O.,\u00a0Piletsky S.A.,\u00a0Sergeeva L.M.,\u00a0El\u2019ska G.V.<\/em> Catalytic molecularly imprinted polymer membranes: Development of the biomimetic sensor for phenols detection. Anal. Chim. Acta, 2010, <strong>659<\/strong>, no.\u00a01\u20132:\u00a0274\u2013279.<\/li>\n<li><em>11. Serheyeva T. A., Pilets\u2019ka O. V., Honcharova L. A., Brovko O. O., Pilets\u2019kyy S. A., Yel\u2019s\u2019ka H. V.<\/em> Sensorna systema na osnovi molekulyarno impryntovanykh polimernykh membran dlya selektyvnoho vyznachennya aflatoksynu \u04121. Ukr. biokhim. zhurn, 2008, <strong>80<\/strong>, no. 3: 84\u201393.<\/li>\n<li><em>12. Sergeyeva T.\u00a0A.,\u00a0Gorbach L.\u00a0A.,\u00a0Slinchenko O.\u00a0A.,\u00a0Goncharova L.\u00a0A.,\u00a0Piletska O.\u00a0V.,\u00a0Brovko O.\u00a0O.,\u00a0Sergeeva L.\u00a0M.,\u00a0El\u2019ska G.\u00a0V.<\/em> Towards development of colorimetric test-systems for phenols detection based on computationally-designed molecularly imprinted polymer membranes. Mater. Sci. Eng. Part C, 2010, Vol.\u00a030:\u00a0431\u2013436.<\/li>\n<li><em>13. Sergeyeva T.\u00a0A.,\u00a0Piletska E.\u00a0V.,\u00a0Piletsky S.\u00a0A.,\u00a0Sergeyeva L.\u00a0M.,\u00a0Brovko O.\u00a0O.,\u00a0El\u2019ska G.\u00a0V.<\/em> Data on structure and recognition properties of the template-selective binding sites in semi-IPN-based molecularly imprinted polymer membranes. Mater. Sci. Eng. Part C, 2008, <strong>28<\/strong>:\u00a01472\u20131479.<\/li>\n<li><em>14. Sergeyeva T. A., Pilets\u2019ka O. V., Brovko O. O., Honcharova L. A., Pilets\u2019kyy S. A., Yel\u2019s\u2019ka H. V.<\/em> Aflatoksynselektyvni molekulyarno impryntovani polimerni membrany na osnovi akrylatpoliuretanovykh napivvzayemopronyknykh polimernykh sitok. Ukr. biokhim. zhurn, 2007, <strong>79<\/strong>, no. 5: 109\u2013115.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>https:\/\/doi.org\/10.15407\/polymerj.38.03.261 Biosensor system for detection of bisphenol\u00a0A in aqueous solutions \u00a0 T.A. Sergeyeva, A.V. Satyr, O.V. Piletska, L.A. Gorbach, \u041e.\u041e. Brovko, A.V. El\u2019skaya &nbsp; Institute of Molecular Biology and Genetics NAS of Ukraine 150 Zabolotnogo str., Kyiv, 03680, Ukraine University of Leicester Leicester\u00a0LE1\u00a07RH, United Kingdom Institute of Macromolecular Chemistry NAS of Ukraine 48, Kharkivske shose, [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"acf":[],"_links":{"self":[{"href":"http:\/\/polymerjournal.kiev.ua\/en\/wp-json\/wp\/v2\/pages\/1223"}],"collection":[{"href":"http:\/\/polymerjournal.kiev.ua\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/polymerjournal.kiev.ua\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/polymerjournal.kiev.ua\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/polymerjournal.kiev.ua\/en\/wp-json\/wp\/v2\/comments?post=1223"}],"version-history":[{"count":4,"href":"http:\/\/polymerjournal.kiev.ua\/en\/wp-json\/wp\/v2\/pages\/1223\/revisions"}],"predecessor-version":[{"id":1732,"href":"http:\/\/polymerjournal.kiev.ua\/en\/wp-json\/wp\/v2\/pages\/1223\/revisions\/1732"}],"wp:attachment":[{"href":"http:\/\/polymerjournal.kiev.ua\/en\/wp-json\/wp\/v2\/media?parent=1223"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}