2019 (3) 6

https://doi.org/10.15407/polymerj.41.03.190

Studying the nanosystem thermosensitive branched polymer/nanogold/chlorine e6 in Hanks’ balanced salt solution

Yu.I. Harahuts, N.V. Kutsevol, N.P. Melnik, O.M. Nadtoka, P.A. Virych

Taras Shevchenko Kyiv National University Department of Chemistry

60, Volodymyrska str., Kyiv, 010601, Ukraine, garaguts.yulia.fox@gmail.com

Polym. J., 2019, 41, no. 3: 190-197.

Section: Medicine polymers.

Language: Ukrainian.

Abstract:

The comparative studies of the behavior of the branched star-like copolymer Dextran-poly-N-isopropylacrylamide, gold nanoparticles in the polymer matrix, and polymer/nanogold/Chlorine e6 nanosystem have been carried out in water and in the Hanks’ balanced salt solution, used for testing on cell cultures, in the physiological temperature range. It has been shown that nanocomposite dimensional parameters do not undergo significant changes in Hank’s balanced salt solution in comparison with aqueous solutions. The size of gold nanoparticles doesn’t change in the temperature region studied. However, a slight aggregation process in nanosystem has been registered at the presence of photosensitizer. That was caused by the change in the hydrophilic-hydrophobic balance of the polymer matrix. It confirmed the interaction of Chlorine e6 with polymer-nanocarrier. This conclusion is in agreement with the results of UV-visible spectroscopy.

The photodynamic activity of the polymer/nanogold/Chlorine e6 nanosystem has also been tested in vitro. The efficiency of the nanocomposite was not much different in comparison with the efficiency of free Chlorine e6 at the same concentration. Obviously, the aggregation processes and the compaction of the polymer-nanocarrier after LCST have resulted in blocking of the photosensitizer.

Key words: thermosensitive copolymer, Dextran-poly-N-isopropylacrylamide, gold nanoparticles, Chlorine e6, photodynamic therapy.

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