2020 (2) 7

https://doi.org/10.15407/polymerj.42.02.136

DEVELOPMENT OF COMPOSITE MATERIALS BASED ON POLYURETHANE UREAS WITH FRAGMENTS OF A COPOLYMER OF POLY(VINYL BUTYRAL, VINYL ACETATE AND VINYL ALCOHOL) AND LYSOZYME

K.V. Stashenko,

Institute of Macromolecular Chemistry NAS of Ukraine, 48, Kharkivske shose, Kyiv, 02160, Ukraine,

e-mail: katuwka2014@gmail.com

ORCID: 0000-0003-1480-1581

T.V. Vislohuzova,

Institute of Macromolecular Chemistry NAS of Ukraine, 48, Kharkivske shose, Kyiv, 02160, Ukraine,

ORCID: 0000-0002-4071-4329

N.A. Galatenko,

Institute of Macromolecular Chemistry NAS of Ukraine, 48, Kharkivske shose, Kyiv, 02160, Ukraine,

e-mail: politoks@merlin.net.ua

ORCID: 0000-0002-5961-5750

R.A. Rozhnova,

Institute of Macromolecular Chemistry NAS of Ukraine, 48, Kharkivske shose, Kyiv, 02160, Ukraine,

e-mail: rozhnovarita@gmail.com

ORCID: 0000-0003-3284-3435

Polym. J., 2020, 42, no. 2: 136-143.

Section: Medical polymers.

Language: Ukrainian.

Abstract:

PUU was obtained on the basis of DPP, GMDA and PVB copolymer for different ratios of DA to PVB as 30:70; 50:50; 70:30 and a number of composite materials with lysozyme were obtained (enzyme content 1 wt.%). The data of IR-spectroscopy studies confirm that the immobilization of lysozyme occurs due to intermolecular hydrogen bonds (physical immobilization). It is established that with increasing amount of PVB copolymer in the structure of polymeric materials, there is an increase in tensile strength and elongation at break. The tensile strength of a series of PUU with increasing PVB are in the range of 1.74–2.29 MPa, and the indices of the elongation at break are in the range of 97–204 %. According to the results of physical-mechanical studies, the introduction of the enzyme into the PUU leads to an improvement of their properties. For compositions with Lysozyme, the tensile strength is 1.43–2.84MPa and the elongation at break is 104–212 %. The intrinsic viscosity of the polymers is in the range 0.46–0.97 dl/g. For PUU, synthesized at a percentage of GMDA:PVB of 70:30 and 30:70, the introduction of lysozyme leads to a slight decrease in the glass transition temperature compared to samples without lysozyme, for PUU synthesized at a percentage of GMDA:PVB of 50:50, leads to increase the glass transition temperature. It was established that the systems under study are single-phase.

Key words: polyurethane urea, copolymer poly(vinyl butyral, vinyl acetate with vinyl alcohol), lysozyme, biologically active coating.

References

1. Ito Y., Iguchi Y., Imanishi Y. Synthesis and non-thrombogenicity of heparinoid polyurethaneureas. Biomaterials, 1992, 13, no. 3: 131–135. https://doi.org/10.1016/0142-9612(92)90060-2
2. Groth Th., Campbell E., Herrmann K. [et al.] Application of enzyme immunoassays for testing haemocompatibility of biomedical polymers. Biomaterials, 1995, 16, no. 13: 1009–1015. https://doi.org/10.1016/0142-9612(95)94909-5
3. Biologicheski aktivnye perevyazochnye sredstva v kompleksnom lechenii gnojno-nekroticheskikh ran. Metodicheskie rekomendaczii no. 2000/56. Pod red. V.D. Fedorova i I.M. Chizha. Moskva, 2000: 36.
4. Romanovskaya I. I. The potential wound coating with trypsin immobilized in modified poly-N-vinylpyrrolidone. Reports of the National Academy of Sciences of Ukraine, 2009, no. 9: 182–187.
5. Makeieva L.V., Gladyr I.I., Roznova R.A., Galatenko N.A., Zakashun T.U., GryzenkoV.P. Bioactive polyurethane ureas with physically immobilized folate-ferrocene conjugate. Reports of the National Academy of Sciences of Ukraine, 2014, no. 10: 131–135. https://doi.org/10.15407/dopovidi2014.10.131
6. Galatenko N. A., Rozhnova R.A., Kulyesh D.V., Andrushina O.S., Demchenko I.B. A new polymeric material with prolonged action for treatment of wounds and burns. Reports of the National Academy of Sciences of Ukraine, 2013, no. 6: 145–151.
7. Galatenko N.A., Roznova R.A., Kulyesh D.V., Rudenchyk T.V., Nechayeva L.Yu., Kebuladze I.M, Popova N.M. Polyurethane ureas with biological activity as materials for drainage in abdominal. Plastic, reconstructive and aesthetic surgery. 2018, no. 1–2: 71–78.
8. Rudenchyk T.V., Roznova R.A., Galatenko N.A., Kiselova T.O. Biocompatible medical film materials. Patent of Ukraine for utility model 133179 Publ. 25.03.2019.
9. Yaminsky I. Bacteria-defeating protein. Popular science physical and mathematical journal «Quantum». 2001, no. 3: 13–15.
10. Gregirchak N.M., Antoniuk M.M. Immobilized enzymes and cells in biotechnology: A summary of lectures for students. special “Industrial Biotechnology” den. and behind forms of teaching. K.: NUKHT, 2011, 59.
11. X. Zhang, M. Sun, Q.Y. Wang Screening condition for lysozyme production of marine bacteria S-12-86. J. Fishery Sci. China, 2007, 3, no. 14: 425–429.
12. Becker G., Berger V., Domske G. Organikum. Workshop on Organic Chemistry in 2 volumes. M.: Mir. (Rus) 1979, 2: 900.